Researcher

The Department of Immunology, Genetics and Pathology at Uppsala University has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: cancer precision medicine, cancer immunotherapy, genomics and neurobiology, molecular tools and functional genomics, neuro-oncology and neurodegeneration and vascular biology. Department activities are also integrated with the units for Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 500 million, out of which more than half is made up of external funding. The staff amounts to approximately 345 employees, out of which 100 are PhD-students, and there are in total more than 700 affiliated people. Feel free to read more about the department's activities here: www.uu.se/en/staff/department/immunology-genetics-and-pathology

Duties:
Establishment, application and improvement of the following methods:

• Zebrafish handling with embryo injections
• Dissociation of fish/embryos/tissue
• RNA isolation and qRT-PCR
• Single-cell sequencing sample preparation
• Bioinformatic analysis / biological interpretation of sequencing results
• Confocal microscopy and analysis (confocal, fluorescent brightfield, Fiji, IMARIS)
• Usage and management of specific vascular transgenic zebrafish and mouse models
• Visualization of blood and lymphatic endothelial cells, vessels, filopodia
• Crossings and stable line generations of zebrafish
• Generation of transgenic lines, CRIPS mutants 
• Molecular biology (cloning, gel electrophoresis, western blotting, cloning, RT-qPCR)
• Microinjection and microdissections (including lymphangiogram and angiogram).
• Histological techniques (immunohistochemistry and in situ hybridisation)

Requirements
The candidate is required to have a PhD degree in Biology, with good knowledge of embryogenesis and transcriptome analysis, and previous postdoctoral experience working with zebrafish and lymphangiogensis are merit.
Competitive CV marked by attendance to conferences presentations, first-author published or pre-prints papers.

About the employment
The employment is limited in time, 5 months with the possibility of extension. The scope is full-time. Access by agreement. Location: Uppsala

Upplysningar om anställningen lämnas av: Kaska Koltowska, kaska.koltowska@igp.uu.se

Välkommen med din ansökan senast den 2 July 2024, UFV-PA 2024/2179.