Researcher in vascular biology - Hiring in process/Finished, not possible to apply

The Department of Immunology, Genetics and Pathology at Uppsala University has a broad research profile with strong research groups focused on, among other things, cancer, autoimmune and genetic diseases. One of the basic ideas at the department is to stimulate translational research and thus a closer collaboration between medical research and healthcare. Research is conducted in medical and clinical genetics, clinical immunology, pathology, neurooncology, vascular biology, radiation science and molecular tools. Parts of the business are also integrated with the departments of oncology, clinical genetics, clinical immunology, clinical pathology and hospital physics at the University Hospital. The department has teaching assignments in a number of program programs and master's programs within the Faculty of Medicine as well as a number of programs in the field of technical-natural science. The department has a turnover of around SEK 450 million, of which just over half is external research grants. The number of employees is about 500, of which about 100 are doctoral students, and there are a total of over 700 active in the workforce. Please read more about the department's activities here: www.igp.uu.se.

Read more about our benefits and what it is like to work at Uppsala University

Duties
Molecular biology methods such as DNA/RNA extraction, plasmid and Lentivirus production, viral transduction of mouse and human cells, qPCR, gel electrophoresis, Chromatin Immunoprecipitation (ChIP), siRNA transfection and Western blot. In vitro cell cultures of both immortalized and primary cells: specifically endothelial cells from mouse and humans, mouse embryonic fibroblasts and mouse stem cells, Human inducible-Pluripotent Stem Cells (iPSCs) production and differentiation towards endothelial cells (PBMCs blood extraction, Sendai Virus transduction, iPSCs colonies isolation, verification, expansion, and endothelial differentiation). 3D angiogenesis and migration assays, invasion assay, 3D cell and stem cell cultures, cell cultures in flow chambers, live-imaging of cell cultures, calcium oscillation imaging in cell cultures, in vitro permeability assay, flow cytometry, tissue sample processing (high expertise in brain, retina and lungs), immunohistochemistry, microscopy (fluorescence epi and confocal microscopy). Human inducible-Pluripotent Stem Cells (iPSCs) production and differentiation towards endothelial cells (PBMCs blood extraction, Sendai Virus transduction, iPSCs colonies isolation, verification, expansion, and endothelial differentiation). Image analysis (ImageJ, IMARIS) and scientific related software (KNIME, Adobe Illustrator, Prism, Mendeley, and Snap Gene). Bioinformatic basic Tools (TIMER platform, TCGA, CCLE database). Teaching (max 20%) and supervision of students.

Requirements
Doctor’s degree in a topic of relevance for the position and long-time experience in vascular biology and immunology. Knowledge in biochemistry, production of iPS cells and disease models of cerebral cavernous malformations (CCM) is required. Genuine knowledge in live cell imaging, flow chamber experiments, 3D cell culture systems (cell spheroids and co-culture systems) and advanced microscopy. Excellent skills in writing and communicating in English is required.

Additional qualifications
Knowledge and experience of iPScell ​​production.

About the employment
The employment is a temporary position, 12 months with the possibility to prolong another 12 months. Scope of employment 100 %. Starting date March 1st 2022 Placement: Uppsala.

For further information about the position, please contact: Peetra Magnusson,
Phone: 0046 18 471 4886, peetra.magnusson@igp.uu.se.

Please submit your application by December 22 2021, UFV-PA 2021/4379.

Are you considering moving to Sweden to work at Uppsala University? Find out more about what it´s like to work and live in Sweden.