Researcher - Hiring in process/Finished, not possible to apply

The Department of Immunology, Genetics and Pathology at Uppsala University (www.igp.uu.se) has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: medical and clinical genetics, clinical immunology, pathology, neuro-oncology, vascular biology, radiation science and molecular tools. Department activities are also integrated with the units for
Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at
Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and in a number of educations at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 440 million, out of which more than half is made up of external funding. The staff amounts to approximately 370 employees, out of which 100 are PhD-students, and there are in total more than 750 affiliated people.

Project description: The applicant will be responsible for a project concerning analysis of blood vessel leakage in normal and tumor-bearing adult mice. A range of mouse strains will be analyzed. The project includes analysis with advanced microscopy, including two-photon and confocal microscopes, of the vascular tree in different organs such as the skin, the trachea and the diaphragm. Various treatments are performed by delivery of stimulating or inhibitory substances, locally or systemically into mice with fluorescent substances in the circulation, supplied by cannulation of the tail vein. Tumor cells are injected intradermally and allowed to grow into an established tumor. Microscopy is performed on the living tissue, with or without tumor, followed by image analysis (ImageJ, Volocity, Imaris or proprietary programs) and statistical evaluation. The fixed tissue can later be analyzed in the confocal microscope.
It is also part of the project to take biopsies and determine the genotype in order to maintain the different recombinant mouse strains. Furthermore, analysis of endothelial cells in culture is included, purified from mouse tissue or purchased. The cells are examined for protein expression and ability to respond to stimuli in various cell biological assays. Isolated endothelial cells are also analyzed for transcript levels by single cell RNA sequencing. The applicant is overall responsible for the detailed planning and execution of the project as well as problem solving. The project is concluded through data compilation for oral and written presentations.

Qualifications:

• Doctoral degree and postdoctoral research training at an international, high-quality level on a subject of relevance for the project.
• Considerable experience of and skill in animal experimentation, genotyping, injections of various kinds, dissection of organs (such as retina), tissue sectioning, and immunostaining. Considerable experience and skill in advanced microscopy using 2-photon and confocal microscopes, and image analysis and statistical analysis.
• Considerable experience and skill in biochemical and cell biology analysis methods (immunoprecipitation, immunoblotting, analysis of various cell reactions).
• Considerable ability to organize the work on the project, to self-motivate and solve problems.
• Documented experience and skill in oral and written presentation in English.

Salary: Individual salary.

Starting date: 01-08-2021.

Type of employment: Permanent position.

Scope of employment: 100 %

For further information about the position please contact: Lena Claesson-Welsh, professor. Tel. +46(0)70-167 92 60, e-mail: lena.welsh@igp.uu.se. 

Please submit your application by 17 of June 2021, UFV-PA 2021/2246.

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