PhD position in molecular biology (protein synthesis) - Hiring in process/Finished, not possible to apply

The Department of Cell and Molecular Biology is one of the most international, interdisciplinary and prominent biomolecular centers in Europe. The department consists of six research programs with approximately 130 employees. The advertised position is part of Prof. Suparna Sanyal's research group at the program of molecular biology (https://www.icm.uu.se/molecular-biology/sanyal-lab/)

Overall Description
The ribosome is the cellular protein factory, which translates genetic codes from messenger RNA (mRNA) into proteins. Protein synthesis also requires many translation factors, which play important roles in the process. In our lab, we try to understand how the ribosome and the translation factors work, how they perform the various steps of protein synthesis. This project aims to explore the mechanisms of protein synthesis in the cold bacteria that live between 0 - 15 ° C.

Work Description
As part of an active research group, you should conduct research to understand the molecular mechanisms of protein synthesis in cold bacteria Pseudoalteromonas haloplanktis TAC125. The assignment involves experimental design, execution, data collection and state-of-the-art analysis of the data. You will perform experiments independently but also interact with other members of the Sanyal group. Specific tasks include (but are not limited to)

• Purification of ribosomes from Pseudoalteromonas haloplanktis TAC125
• Cloning and purification of all translation factors from P. Haloplanktis TAC125
• Fast kinetic analysis to study the mechanisms of different steps of translation
• Biochemical data analysis
• Documentation and dissemination of the knowledge in writing and oral forms

The doctoral student shall primarily devote himself/herself to his/her postgraduate studies full-time within the agreed research period. Other service at the department, which relates to education and administrative work, will not exceed 20%.

Requirements

• Master's degree in molecular biology / molecular biotechnology / biochemistry or equivalent
• Experience in molecular cloning, protein overexpression and purification
• Knowledge of biochemistry (especially enzyme kinetics)
• Basic knowledge of kinetic data analysis programs (e.g. Origin)
• Excellent communication skills in English

For the appointment of the PhD student, the candidate must be accepted or admitted to postgraduate education.

Merits will be given to the candidate with

• Experience in the production and purification of ribosomes.
• Experience of using stopped-flow or quench flow
• Experience of using HPLC

Application:

Has to be written in English containing:

• A personal letter describing yourself, your research interest and your experiences (max 2 pages)
• CV containing education and other qualifications as well as description of relevant knowledge and techniques based on the qualifications. List of publications.
• Certified copies of diplomas and a copy of relevant exam work or publication.
• Two reference persons with contact information. It should show your relationship with the people.

Rules governing Ph.D. candidates are set out in the Higher Education Ordinance Chapter 5, §§ 1-7 and in Uppsala university's rules and guidelines http://regler.uu.se/search/?hits=30&languageId=1&search-language_en=English

Uppsala University strives to be an inclusive workplace that promotes equal opportunities and attracts qualified candidates who can contribute to the University’s excellence and diversity. We welcome applications from all sections of the community and from people of all backgrounds.

Salary: According to local agreement (Local collective agreement for doctoral students and amanuenses).

Starting date:  As soon as possible, or otherwise agreed on.

Scope of employment: fulltime, 100%

Information about the employment can be asked from Suparna Sanyal, tel 018-471 4220, e-mail suparna.sanyal@icm.uu.se. See also the department's website: www.icm.uu.se

Welcome with your application by January 31st, 2019, UFV-PA 2019/169.

Are you considering moving to Sweden to work at Uppsala University? If so, you will find a lot of information about working and living in Sweden at www.uu.se/joinus. You are also welcome to contact International Faculty and Staff Services at ifss@uadm.uu.se.